Complete genome analysis of African swine fever virus responsible for outbreaks in domestic pigs in 2018 in Burundi and 2019 in Malawi

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dc.contributor.author N. Hakizimana, Jean
dc.contributor.author B. Ntirandekura, Jean
dc.contributor.author Yona, Clara
dc.contributor.author Nyabongo, Lionel
dc.contributor.author Kamwendo, Gladson
dc.contributor.author L. C. Chulu, Julius
dc.contributor.author Ntakirutimana, Désiré
dc.contributor.author Kamana, Olivier
dc.contributor.author Nauwynck, Hans
dc.contributor.author Misinzo, Gerald
dc.date.accessioned 2021-09-01T08:16:06Z
dc.date.available 2021-09-01T08:16:06Z
dc.date.issued 2021-08-17
dc.identifier.uri https://repository.rsif-paset.org/xmlui/handle/123456789/117
dc.description Journal Article en_US
dc.description.abstract Several African swine fever (ASF) outbreaks in domestic pigs have been reported in Burundi and Malawi and whole-genome sequences of circulating outbreak viruses in these countries are limited. In the present study, complete genome sequences of ASF viruses (ASFV) that caused the 2018 outbreak in Burundi (BUR/18/Rutana) and the 2019 outbreak in Malawi (MAL/19/Karonga) were produced using Illumina next-generation sequencing (NGS) platform and compared with other previously described ASFV complete genomes. The complete nucleotide sequences of BUR/18/Rutana and MAL/19/Karonga were 176,564 and 183,325 base pairs long with GC content of 38.62 and 38.48%, respectively. The MAL/19/Karonga virus had a total of 186 open reading frames (ORFs) while the BUR/18/Rutana strain had 151 ORFs. After comparative genomic analysis, the MAL/19/Karonga virus showed greater than 99% nucleotide identity with other complete nucleotides sequences of p72 genotype II viruses previously described in Tanzania, Europe and Asia including the Georgia 2007/1 isolate. The Burundian ASFV BUR/18/Rutana exhibited 98.95 to 99.34% nucleotide identity with genotype X ASFV previously described in Kenya and in Democratic Republic of the Congo (DRC). The serotyping results classified the BUR/18/Rutana and MAL/19/Karonga ASFV strains in serogroups 7 and 8, respectively. The results of this study provide insight into the genetic structure and antigenic diversity of ASFV strains circulating in Burundi and Malawi. This is important in order to understand the transmission dynamics and genetic evolution of ASFV in eastern Africa, with an ultimate goal of designing an efficient risk management strategy against ASF transboundary spread. en_US
dc.publisher Tropical Animal Health and Production en_US
dc.subject African swine fever virus, Asfarviridae, Burundi, Domestic pig, Malawi, Whole-genome sequencing en_US
dc.title Complete genome analysis of African swine fever virus responsible for outbreaks in domestic pigs in 2018 in Burundi and 2019 in Malawi en_US
dc.type Article en_US

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